How are Stem Cells Harvested: Exploring Various Methods

May 31, 2023

Understanding how stem cells are harvested is crucial for those seeking non-surgical approaches to healing, particularly for individuals with chronic pain. This article will investigate the different means and sources of obtaining stem cells for non-surgical healing, particularly those with chronic pain.

Bone marrow aspiration is a method of harvesting hematopoietic stem cells from bone marrow, which may be used for regenerative medicine due to their potential. Next, we’ll discuss adipose-derived mesenchymal stromal/stem cells and examine their potential applications alongside concerns over contamination risk and processing techniques.

Moving forward, we will analyze umbilical cord blood stem cells while addressing limitations associated with using this source for therapy. Lastly, our discussion will cover dental pulp-derived stem cells by highlighting their applications in dentistry and emphasizing the need for additional research on their therapeutic potential.

By the end of this post, you should have a comprehensive understanding of how stem cells are harvested from different sources and be able to make informed decisions about your own treatment options.

Table of Contents:

Bone Marrow Stem Cells Harvesting

At Total Stem Cell, our main focus is on the utilization of bone marrow stem cells for regenerative medicine purposes. Bone marrow stem cells are considered one of the safest options available for this type of therapy. Stem cells derived from bone marrow have shown great potential in various medical applications, including tissue repair, organ regeneration, and immune system enhancement.

To obtain these valuable stem cells, we perform a minimally invasive procedure called bone marrow aspiration. Before the procedure begins, patients receive local anesthesia to ensure their comfort. During bone marrow aspiration, a needle is carefully inserted into the bone to extract liquid bone marrow. This liquid bone marrow is rich in hematopoietic stem cells (HSCs), which are the main type of stem cells found in bone marrow. These HSCs have the unique ability to differentiate into various types of blood cells, making them particularly valuable for treating blood disorders, such as leukemia or aplastic anemia.

By using bone marrow aspiration, we can acquire a concentrated sample of HSCs from the patient’s own body. This minimally invasive approach not only reduces the risk of complications but also avoids the ethical concerns associated with other types of stem cell sources. Moreover, using a patient’s own bone marrow cells eliminates the potential for rejection or immune system reactions, as the cells are a perfect match for their body.

At Total Stem Cell, our mission is to harness the power of bone marrow stem cells and provide safe and effective regenerative medicine treatments for our patients.

Hematopoietic Stem Cells and Their Potential in Regenerative Medicine

HSCs have demonstrated their efficacy in treating a variety of conditions, from chronic pain to autoimmune diseases and even certain types of cancer.

These multipotent cells can differentiate into different blood cell types which play crucial roles in our body’s healing processes.

The Process of Bone Marrow Aspiration

  • Patient receives local anesthesia to numb the area.
  • A needle is carefully inserted through the skin and into the bone.
  • Liquid bone marrow containing HSCs is extracted using a syringe.
  • The sample undergoes processing to isolate desired stem cells for therapy.

For more information on the benefits of bone marrow stem cells, check out Healthline.

Adipose-Derived Mesenchymal Stromal/Stem Cells

A popular way to get stem cells comes from adipose-derived mesenchymal stromal/stem cell isolation. These cells are derived from liposuction aspirates during cosmetic surgery procedures.

Potential applications for adipose-derived stem cells

  • Need to regenerate tissue or heal a wound? Adipose-derived stem cells may help.
  • Got osteoarthritis? These stem cells could be a treatment option.
  • Dealing with an inflammatory disease? Adipose-derived stem cells might be able to help manage it.
  • Have cardiovascular disease? These stem cells could be used in therapy.

Concerns over contamination risk and processing techniques

One of the key worries about adipose-derived stem cells is that contamination may occur during collection, as well as differences in processing methods which could influence cell viability. To ensure patient safety, it’s essential to establish strict guidelines for harvesting and handling these valuable resources. (source)

Umbilical Cord Blood Stem Cells

Umbilical cord blood is full of stem cells that can be harvested without harming the mother or newborn, making it a popular choice for stem cell therapy.

However, umbilical cord blood has certain drawbacks, such as the limited amount of cells in each donation which could necessitate multiple donations for successful treatment.

Limitations associated with using umbilical cord blood for therapy

  • Cell quantity: The number of stem cells obtained from a single umbilical cord blood donation may not be sufficient for some treatments, necessitating additional collections.
  • Cryopreservation: Umbilical cord blood stem cells must be stored in specialized facilities at ultra-low temperatures to preserve their viability, which can add logistical challenges and costs.
  • Ethics: Although harvesting umbilical cord blood is noninvasive and poses no risks to the mother or baby, there are still ethical considerations surrounding consent and potential commercialization of this resource.

Despite their drawbacks, umbilical cord blood stem cells remain a highly beneficial asset for medical investigation and treatment.

Dental Pulp-Derived Stem Cells

Dental pulp-derived stem cells hold promise as a potential solution for noninvasive dental treatments. These stem cells have the ability to differentiate into different types of cells, which makes them well-suited for repairing damaged teeth and supporting structures.

However, it is important to note that further studies are needed to fully understand and ensure the safety and effectiveness of these stem cells. Currently, there are challenges regarding the isolation process and long-term storage capabilities of dental pulp-derived stem cells that need to be addressed.

While dental pulp-derived stem cells show potential, it is crucial to continue researching and evaluating their applications in order to unlock their full potential as a treatment option for dental conditions.

Fortunately, the use of dental pulp-derived stem cells can avoid more intrusive treatments.

  • Regenerative medicine applications: Dental pulp-derived stem cells offer an attractive alternative to more invasive methods for issues such as tooth decay, gum inflammation, and periodontal disease.
  • Challenges facing this source: Further research is needed to optimize the therapeutic potential of dental pulp-derived stem cells.
  • Learn more: Check out this study for more information on the potential of dental pulp-derived stem cells in regenerative medicine.

FAQs in Relation to How Are Stem Cells Harvested

How are stem cells harvested?

Stem cells are harvested from various sources, including bone marrow, adipose tissue (fat), umbilical cord blood, and dental pulp, with the process varying depending on the source.

How are stem cells sourced?

Stem cells can be sourced from different tissues in the body such as bone marrow, adipose tissue, umbilical cord blood, and dental pulp.

Where do stem cells come from and how are they harvested?

Stem cells originate from various tissues within the body and can be obtained through processes like bone marrow aspiration, liposuction, or collecting umbilical cord blood after childbirth.

What are the three ways to obtain stem cells?

The three common methods to obtain stem cells include bone marrow aspiration, adipose tissue extraction, and umbilical cord blood collection.


How do we get stem cells? There are four ways: bone marrow, adipose tissue, umbilical cord blood, and dental pulp.

  • Bone marrow aspiration involves extracting hematopoietic stem cells from the bone marrow.
  • Adipose-derived mesenchymal stromal/stem cells are obtained through liposuction.
  • Umbilical cord blood is collected during childbirth.
  • Dental pulp-derived stem cells come from extracted teeth.

Each source has potential applications in regenerative medicine and dentistry, but there are concerns over contamination risk and processing techniques for adipose-derived stem cells, and limitations associated with using umbilical cord blood for therapy.

If you struggle with chronic pain and would like to incorporate stem cell therapy into your recovery, please contact Total Stem Cell today and see how we can help you!